ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader.

Laura de Diego-García,Álvaro Sebastián-Serrano,Carolina Bianchi,Caterina Di Lauro,Miguel Díaz-Hernández

PURINERGIC SIGNALING: METHODS AND PROTOCOLS（2020）

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摘要

Imbalance in extracellular ATP levels in brain tissue has been suggested as a triggering factor for several neurological disorders. Here, we describe the most sensitive and reliable technique for monitoring the ATP levels in mice cerebrospinal samples collected by cisterna magna puncture technique and quantified using a microplate reader.

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关键词

ATP,Cerebrospinal fluid,In vivo,Luciferase,Luciferin,Microplate reader,Neurological disorders

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