Infrared Nanoscopy Of Alive Biological Cell Surfaces
2019 44TH INTERNATIONAL CONFERENCE ON INFRARED, MILLIMETER, AND TERAHERTZ WAVES (IRMMW-THZ)(2019)
Abstract
We report progress towards sealing alive cells in their native, wet environment by single-layer graphene, where graphene stabilizes the cell by tightly conforming with its surface and thus enables topographic AFM maps of the cell's surface. Furthermore, graphene's transparency to infrared near-fields enables local chemical recognition, at nominally 20-nm spatial resolution, by scattering scanning mid-infrared microscopy (s-SNOM) and spectroscopy (nano-FTIR). here we demonstrate first infrared nanoscopy results of the surface of native E. Coli cells that show spatial and spectral contrasts.Our study opens a new door into characterizing subcellular structures and components of cells and of fresh tissue sections. Infrared nanoscopy is certainly free of any need for labelling as in super-resolution fluorescence microscopy, and free of fixation and metallization procedures as in electron microscopy that kill and distort cells.
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Key words
infrared nanoscopy,alive biological cell surfaces,native environment,wet environment,single-layer graphene,topographic AFM maps,local chemical recognition,scanning mid-infrared microscopy,native E. Coli cells,subcellular structures,superresolution fluorescence microscopy,distort cells,s-SNOM,nanoFTIR spectra,fresh tissue
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