O4A.1 Night shiftwork, dna methylation, and telomere length in female nurses

Introduction Studies on female nurses have reported a higher breast cancer risk among night shift (NS) workers, without a clear understanding of the underlying biological mechanisms. Aim To assess the association between night shiftwork and molecular alterations potentially related to a higher carcinogenic risk, in details: DNA methylation of estrogen receptor (ER-Alpha, ER-Beta) and tumor suppressor (BRCA1, BRCA2, p53, p16) genes, global DNA methylation estimated on repeated elements (LINE-1, Alu), and telomere length (TL). Methods 46 female nurses who had been working in NS for at least two years in a Hospital in Milan, Italy, were matched by age (30–45 years) and length of service (at least 5 years) with 51 female colleagues not working in NS. Each subject was administered a structured questionnaire and withdrawn a 12 ml blood sample. We applied linear regression models adjusted for age, BMI, smoking habit, parity, and oral contraceptive use. Results Currently working in NS (yes/no) was associated with hypomethylation of ER-Alpha (β: −1.635, 95% CI: −2.715; −0.554). When examining both current and former NS workers, the number of years (NY) in NS was associated with hypermethylation of Alu (β: 0.078, 95% CI: 0.016; 0.138). After graphical inspection of the association between NYNS and TL, we classified the study population according to NS duration ( Conclusions Our findings show NS-associated epigenetic alterations that might be involved in processes such as cellular aging, genomic instability, and cancer development.