Biomimetic Synthesis Of All-Inclusive Organic-Inorganic Nanospheres For Enhanced Electrochemical Immunoassay

A friendly biomimetic process was adopted for the mild preparation of "all-inclusive" organic-inorganic nanospheres, which effectively integrate biorecognition function and signal amplification function. The resulted Ca-3(PO4)(2)-Ab(2)-BSA nanospheres were employed as signal labels for enhancing detection of nuclear matrix protein 22 (NMP 22). The fabricated electrochemical immunosensor exhibited a linear range (0.08-77.00 U/mL) and an ultralow limit of detection (0.01 U/mL) towards NMP 22, which can be taken as a promising tool for clinical diagnosis of bladder cancer. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.