Calcium signalling in mammalian cell lines expressing wild type and mutant human α1-Antitrypsin

SCIENTIFIC REPORTS(2019)

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摘要
A possible role for calcium signalling in the autosomal dominant form of dementia, familial encephalopathy with neuroserpin inclusion bodies (FENIB), has been proposed, which may point towards a mechanism by which cells could sense and respond to the accumulation of mutant serpin polymers in the endoplasmic reticulum (ER). We therefore explored possible defects in Ca 2+ -signalling, which may contribute to the pathology associated with another serpinopathy, α 1 -antitrypsin (AAT) deficiency. Using CHO K1 cell lines stably expressing a wild type human AAT (MAAT) and a disease-causing polymer-forming variant (ZAAT) and the truncated variant (NHK AAT), we measured basal intracellular free Ca 2+ , its responses to thapsigargin (TG), an ER Ca 2+ -ATPase blocker, and store-operated Ca 2+ -entry (SOCE). Our fura2 based Ca 2+ measurements detected no differences between these 3 parameters in cell lines expressing MAAT and cell lines expressing ZAAT and NHK AAT mutants. Thus, in our cell-based models of α1-antitrypsin (AAT) deficiency, unlike the case for FENIB, we were unable to detect defects in calcium signalling.
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Biological techniques,Medical research,Science,Humanities and Social Sciences,multidisciplinary
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