Development of a method to measure laccase activity on methoxyphenolic food ingredients and isomers
International Journal of Biological Macromolecules(2020)
Abstract
•A chronometric method for measuring laccase activity has been developed.•The method is applicable to a wide variety of methoxyphenols as substrates of laccase.•It measures the time “τ” the enzyme system takes to consume a certain amount of ascorbic acid.•Methoxyphenols as laccase substrates are kinetically characterized.
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Key words
Laccase,Trametes versicolor,Chronometric method,Methoxyphenols,Guaiacol,2,6-Dimethoxyphenol
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