Comparison of different commercially available enzyme-linked immunosorbent assays with immunofluorescence test for the detection of Phase II IgG and IgM antibodies to Coxiella burnetii .
JOURNAL OF CLINICAL MICROBIOLOGY(2020)
摘要
Several commercially available enzyme-linked immunosorbent assays (ELISAs) for the detection of phase II IgG or IgM antibodies against Coxiella burnetii were compared. In addition, an indirect immunofluorescence test was used as a confirmation test. In all, 70 serum samples for IgG and 43 serum samples for IgM were tested. The ELISAs showed large differences in sensitivity and specificity, which led to a partially high ratio of false-negative determinations. The most convincing test was PanBio from Abbott, which unfortunately can only test IgG but not IgM.
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关键词
Coxiella burnetii,Q fever,enzyme-linked immunosorbent assay,serological diagnosis,zoonosis
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