Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application

Background Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses. Results This study aimed to identify and characterize the GmRD26 promoter (p GmRD26 ), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of p GmRD26 :: GUS (2.054 bp) and two promoter modules, p GmRD26A :: GUS (909 pb) and p GmRD26B :: GUS (435 bp), controlling the expression of the β-glucuronidase ( uidA ) gene. Analysis of GUS activity has demonstrated that p GmRD26 and p GmRD26A induce strong reporter gene expression, as the p AtRD29 positive control promoter under ABA and PEG treatment. Conclusions The full-length promoter p GmRD26 and the p GmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that p GmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses.