Improved Electrophoretic Separation To Assist The Monitoring Of Bcl-Xl Post-Translational Modifications
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(2019)
Abstract
Bcl-x(L) is an oncogene of which the survival functions are finely tuned by post-translational modifications (PTM). Within the Bcl-2 family of proteins, Bcl-x(L) shows unique eligibility to deamidation, a time-related spontaneous reaction. Deamidation is still a largely overlooked PTM due to a lack of easy techniques to monitor Asn -> Asp/IsoAsp conversions or Glu -> Gln conversions. Being able to detect PTMs is essential to achieve a comprehensive description of all the regulatory mechanisms and functions a protein can carry out. Here, we report a gel composition improving the electrophoretic separation of deamidated forms of Bcl-x(L) generated either by mutagenesis or by alkaline treatment. Importantly, this new gel formulation proved efficient to provide the long-sought evidence that even doubly-deamidated Bcl-x(L) remains eligible for regulation by phosphorylation.
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Key words
Bcl-x(L),post-translational modification,deamidation,phosphorylation,electrophoresis
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