Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells.
Methods in molecular biology (Clifton, N.J.)（2020）
Abstract
The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture for the transduction of chimeric antigen receptor T-cells (CAR-T) can be achieved by different methods. This chapter describes LV production by transient transfection, induction of stable packaging cell lines, and induction of stable producer cell lines.
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Key words
Lentiviral vectors,Packaging cells,Producer cells,Serum-free medium,Suspension cells,Transient transfection
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