Pseudidiomarina gelatinasegens sp. nov., isolated from surface sediment of the Terra Nova Bay, Antarctica

Polyphasic taxonomic analysis was performed to characterize a novel bacterium, which was isolated from surface sediment of the Terra Nova Bay, Antarctica, and designated as R04H25(T). The cells of the isolate were Gram-stain-negative, aerobic, nonmotile, slightly curved rods. Growth occurred at 4-42 degrees C, pH 7.0-9.5, and in 1-15% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences indicated that strain R04H25(T) formed an independent lineage within the genus Pseudidiomarina and its nearest neighbours were Pseudidiomarina donghaiensis 908033(T) (98.2%), Pseudidiomarina marina PIM1(T) (98.1%), Pseudidiomarina woesei W11(T) (97.8%), Pseudidiomarina maritima 908087 (97.1%) and Pseudidiomarina tainanensis PIN1(T) (97.0%). The average nucleotide identities between strain R04H25(T) and the nearest neighbours were 76.2-77.7%. The major fatty acids were iso-C-17:0, summed feature 9, iso-C-15:0,C-16:0 and iso-C-11:0 3-OH. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid, three unidentified glycolipids and two unidentified lipids. The predominant respiratory quinone was ubiquinone 8. The genomic DNA G+C content was 48.2 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic results, we propose a novel species named as Pseudidiomarina gelatinasegens sp. nov. in the genus Pseudidiomarina, with the type strain R04H25(T) (=GDMCC 1.1503(T) =KCTC 62911(T)).