A simple method to calculate the degree of polymerization of alginate oligosaccharides and low molecular weight alginates.

This study presents a quick, simple and accurate method to calculate the degree of polymerization (DP) of alginate oligosaccharides (AOS) and low molecular weight alginates from the concentration of reducing sugar determined by 3,5-dinitrosalicylic acid (DNS) assay. 1H NMR spectroscopy, mass spectroscopy (MS) and certified standards were used to verify the accuracy of this method, and the results showed DP calculated from DNS assay agreed with the actual DP. This method has great potential to simplify the process of measuring DP of alginate in lab and thus could be incorporated into various researches on alginates in the future. Moreover, similar method could be applied when studying the DP of other oligosaccharides.