A New Dna Repair-Related Platform For Pharmaceutical Outlook In Cancer Therapies: Ultrashort Single-Stranded Polynucleotides

Thio- and cyano- modified single-stranded poly(dNTP) sequences of different molecular sizes (20-200 n) and the same lengths routine poly(dNTP) and poly(NTP) species were tested for their impact on catalytic activities of beta-like DNA polymerases from chromatin of HL-60, WERI-1A and Y-79 cells as well as for the affinity patterns in DNApol beta-poly(dNTP)/(NTP) pairs, respectively. An essential link between the lengths of ultrashort (50-100 n) single-stranded poly(dNTP) sequences of different structures and their inhibitory effects towards the cancer-specific DNA polymerases beta was found. A possible significance of this phenomenon for both DNA repair suppression in tumors and a consequent anti-cancer activity of the DNA repair related short poly(dNTP) fragments is under discussion.