Multiallelic marker system for traceability of Black Slavonian pig meat

Meat from Black Slavonian pig (Crna slavonska, CS), an autochthonous Croatian breed, is used for the production of high-quality products, which makes it an interesting target for meat fraud. The aim of the study was to develop a set of microsatellite markers suitable for precise genetic traceability of the meat originating from this breed. Initially, 23 microsatellite markers were selected and grouped into three multiplex reactions for the genetic characterization of the seven breeds included in the study; subsequently, a set of eight microsatellite markers for traceability of CS pig was created based on the match probability value. The results indicated that when eight highly polymorphic loci were combined, the chance of finding identical genotype in two random individuals was about three in ten million (10−7). This provided the basis for establishing a reliable genetic traceability system for meat originating from CS pigs.