EFFECT OF gamma-SECRETASE INHIBITOR ON TREG IN PBMC OF RESPIRATORY SYNCYTIAL VIRUS BRONCHIOLITIS CHILDREN

Bronchiolitis is a widespread lower respiratory tract infection in infants and young children, and is closely related to the incidence of asthma, and T regulatory cells (Tregs) play a role in its pathogenesis. Notch signaling pathways are closely related to T cells and play a role in respiratory syncytial virus (RSV) bronchiolitis. In this study, we observed the changes in Treg and the level of FoxP3 mRNA in PBMCs in children with RSV bronchiolitis after blocking Notch signaling pathway by gamma-secretase inhibitor MW167, which provides a new idea for RSV bronchiolitis immunotherapy. The study enrolled 30 patients with RSV bronchiolitis and 25 normal controls. PBMC was separated and divided into a normal control group, an RSV group, and an MW167 group. The percentage of Tregs was detected by flow cytometry, and the level of FoxP3 mRNA was detected by real-time fluorescent quantitative PCR. Compared with the normal group, the percentage of Tregs in the RSV group was decreased, and the levels of FoxP3 mRNA were significantly decreased. Compared with the RSV group, the percentage of Tregs in the MW167 group was increased, and the levels of FoxP3 mRNA were increased. There are changes in the percentage of Tregs and the level of transcription factor FoxP3 in PBMCs of children with RSV bronchiolitis, and gamma-secretase inhibitor MW167 can reverse this change to a certain extent.