Evaluation of safety and probiotic properties of a strain of Enterococcus faecium isolated from chicken bile
Journal of Food Science and Technology(2019)
摘要
Probiotics are important bacteria due to their benefit on human health. In this study, four strains of lactic acid bacteria from chicken bile were isolated and the strain with the best antimicrobial activity was selected for further identification and evaluation on its probiotic traits and safety. The strain was identified as Enterococcus faecium by biochemical characterization and 16S rDNA gene sequencing. The strain, named E. faecium MK-SQ-1, was tolerant to acid (pH 3.0), bile salts (up to 0.3%) or trypsin (up to 0.4%) for 3 h and it was able to survive from high temperature (up to 60 °C) for 15 min. This strain inhibited the growth of Salmonella enteritidis and Staphylococcus aureus intermediately. The genes responsible for virulence including asa1, cylA, efaA, esp, gelE and hyl were absent and the mice administrated orally with a very high dose (2 × 10 9 CFU) of the strain daily for 35 days were not found abnormal. The strain enhanced the serum IgG level and phagocytic index of mice significantly by daily oral administration at a high dose (2 × 10 8 CFU) for 21 days ( p < 0.05). The strain did not have multi-antibiotic resistance and vancomycin resistance. Comprehensive evaluation showed E. faecium MK-SQ-1 could be a candidate as a probiotic strain used in human or animals.
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关键词
Enterococcus faecium
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