Nocardioides Sambongensis Sp. Nov., Isolated From Dokdo Islands Soil

Strain KUDC5002(T) was isolated from soil sampled on the Dokdo Islands, Republic of Korea. This bacterial strain was Gram stain-positive, non-motile, rod-shaped, capable of growing at 25-37 degrees C and pH 5.0-12.0, and showed optimal growth at 30 degrees C and pH 7.0-8.0. Strain KUDC5002(T) could be grown in tryptic soy broth containing less than 7.0% NaCl (w/v). The cell width ranged from 0.5 to 0.6 mu m and length ranged from 0.8 to 1.0 mu m. Strain KUDC5002(T) was catalase- and oxidase-positive. Its genomic G+C content was 72.2 mol%. Its major fatty acids were C-18:1 omega 9c (17.3 %), iso-C-16:0 (16.0 %) and iso-C-17:0 (11.4 %). Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain KUDC5002(T) belongs to the genus Nocardioides and is most closely related to strain Nocardioides humi DCY24(T) (97.0 %). Based on its phenotypic, phylogenetic, genetic and chemotaxonomic features, strain KUDC5002(T) should be considered a novel species in the genus Nocardioides, for which we have proposed the name Nocardioides sambongensis sp. nov. The type strain is KUDC5002T (=KCTC 39855(T)=DSM 106604(T)).