Effect Of Intermittent Hypoxia On Cardiac Muscle Calcium Homeostasis In Experimental Type 1 Diabetes Mellitus

Purpose: In this study; it was investigated the effect of intermittent hypoxia on cardiac phospholamban and CaMKII levels in experimental diabetic cardiomyopathy. Material and method:Wistar albino male rats (n=34) were randomized to four groups: control (C), intermittent hypoxia (IH), diabetes mellitus (DM) and diabetes mellitus + intermittent hypoxia (DM+IH). Injection of streptozotocin (50 mg/kg, i.p.) followed by 250 mg/dL and above blood glucose levels , was accepted as diabetes mellitus. The IH and DM+IH groups were subjected to 6 hours/day hypoxia for 42 days at a pressure corresponding to a height of 3000 m. 24 hours after the IH protocol was completed, the hearts of the animals were removed. Phospholamban and CaMKII were conducted by agarose gel electrophoresis method after polymerase chain reaction. After the images were obtained with a UV camera; band density was determined in the Image J program. The resulting data were compared with the Kruskal Wallis test, multiple comparisons tests, and the Wilcoxon test. Results:When the rates of the increasing weight of the experimental animals were examined, it was observed that the weight increase in the IH group was at most and the DM group was at least. The differences between C and DM (p=0.003), C to DM+IH (p=0.024), IH to DM (p=0.001), IH to DM+IH (p=0.006) groups were statistically meaningful at the end of the experiment. It has not been detected any meaningful difference among the groups of PLB/GAPDH (p=0.294). In terms of CaMKII/GAPDH, a statistically significant difference was found between C and DM; C and DM+IH and IH and DM+IH groups (p lt;0.05). Conclusion:Our study suggests that CaMKII has a role in diabetic cardiomyopathy in detecting differences in CaMKII level. However, changes in the phospholamban have not been detected, but are important in the effects of translational and/or posttranslational levels and in the changes that may occur in protein levels and/or activations.