Cladophora fascicularis Mediated Silver Nanoparticles: Assessment of Their Antibacterial Activity Against Aeromonas hydrophila

This study reports on the bioactive potential of silver nanoparticle synthesized from seaweed, Cladophora fascicularis aqueous extract. C. fascicularis assisted silver nanoparticles (Cf-AgNPs) was characterized by UV–visible spectroscopy, XRD, FT-IR, FESEM, TEM, and EDX analysis. The Cf-AgNPs exhibited a maximum absorption peak at 427 nm. The carboxyl (–C=O) and amine (N–H) groups of C. fascicularis extract is answerable for the synthesis of Cf-AgNPs. The microscopic analysis confirmed that the synthesized Cf-AgNPs are spherical in shape with an average size of 45 nm. The Cf-AgNPs exhibited a dose depended antibacterial activity against Aeromonas hydrophila . Noteworthy bacterial inhibition (19 mm) was observed at 150 µg/ml, whereas, the lowest activity (10 mm) was recorded at 25 µg/ml. Similarly, Cf-AgNPs significantly (P < 0.05) enhanced the protein leakage in A. hydrophila by increasing the membrane permeability. The considerable amount of ROS activity was noticed in the test bacterium A. hydrophila when treated with 150 µg/ml of Cf-AgNPs. The toxicity investigation results revealed that the synthesized Cf-AgNPs were not acutely toxic to Artemia nauplii even at a higher concentration (150 μg/ml). These results underline that Cf-AgNPs effectively inhibited the growth of A. hydrophila and showed the non-toxic effect with outstanding biocompatibility.