Inhibition of Agrobacterium tumefaciens biofilm formation by acylase I-immobilized polymer surface grafting of a zwitterionic group-containing polymer brush

•Acylase I was immobilized on a zwitterionic membrane sheet prepared by grafting.•The prepared sheet had improved hydrophilicity due to the zwitterionic group.•The sheet inhibited Agrobacterium tumefaciens biofilm formation.•Inhibition of biofilm formation only occurred when acylase I was active.•Quorum quenching function by acylase I is important for biofilm inhibition.