Electrochemical detection of lipopolysaccharide based on rolling circle amplification assisted formation of copper nanoparticles for enhanced resistance generation
Sensors and Actuators B: Chemical（2019）
Abstract
•A sandwich impedimetric electrochemical aptasensor was constructed for the determination of LPS.•The poly-T ssDNA sequences from RCA served as specific template for CuNPs formation.•CuNPs@ssDNA catalyzed PCV into insoluble bulky oligomer for enhanced resistance generation.•The aptasensor exhibited good performance for LPS detection with a detection limit of 4.8 fg/mL.
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Key words
Sandwich-type impedimetric aptasensor,Lipopolysaccharide,Rolling circle amplification,Copper nanoparticles,Precipitation
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