Fabrication of a biocompatible and continuous glucose biosensor with the poly(3,4-ethylenedioxythiophene) modified electrode

•A feasible GOx/AuNP/PEDOT(BSA)/Pt biosensor for continuous glucose measurement has been proposed.•The glucose biosensor fabrication relies on a simple two-stage self-assembly process with no crosslink reagents involved.•GOx is exposed on the surface of electrode for easy access by glucose.•The glucose measurement is independent on dissolved oxygen and belongs to the third generation of glucose biosensor.•The glucose biosensor is biocompatible and has exhibited a stable performance in a 7-day continuous measurement.