Preparation, characterization, and evaluation of cytotoxicity, antioxidant, cutaneous wound healing, antibacterial, and antifungal effects of gold nanoparticles using the aqueous extract of Falcaria vulgaris leaves

The new age drugs are nanoparticles of metals, which can combat conditions like wounds and fight human pathogens like bacteria. The aim of the experiment was preparation, characterization, and assessment of cytotoxicity, antioxidant, cutaneous wound healing, antibacterial, and antifungal potentials of gold nanoparticles using the aqueous extract of Falcaria vulgaris leaves (AuNPs@F. vulgaris) under in vitro and in vivo condition. These nanoparticles were characterized by FT-IR, UV, XRD, FE-SEM, TEM, and AFM. The synthesized AuNPs@F. vulgaris had great cell viability dose-dependently (Investigating the effect of the nanoparticles on HUVEC cell line) and indicated these nanoparticles were nontoxic. DPPH free radical scavenging test was done to evaluate the antioxidant potentials, which showed similar antioxidant potentials for AuNPs@F. vulgaris and butylated hydroxytoluene. In part of cutaneous wound healing effect of F. vulgaris, after creating the cutaneous wound, the rats were randomly divided into six groups: untreated control, treatment with Eucerin basal ointment, treatment with 3% tetracycline ointment, treatment with 0.2% HAuCl4 x H2O ointment, treatment with 0.2% F. vulgaris ointment, and treatment with 0.2% AuNPs@F. vulgaris ointment. These groups were treated for 10 days. Use of AuNPs@F. vulgaris ointment in the treatment groups substantially decreased (p <= 0.01) the wound area, total cells, neutrophil, and lymphocyte and remarkably raised (p <= 0.01) the wound contracture, hydroxyl proline, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate compared to other groups. In antimicrobial part, MIC, MBC, and MFC were specified by macro-broth dilution assay. AuNPs@F. vulgaris revealed higher antibacterial and antifungal properties than many standard antibiotics (p <= 0.01). Also, AuNPs@F. vulgaris prevented the growth of all bacteria at 2-8 mg/ml concentrations and removed them at 2-16 mg/ml concentrations (p <= 0.01). In case of antifungal potentials of AuNPs@F. vulgaris, they inhibited the growth of all fungi at 2-4 mg/ml concentrations and destroyed them at 2-8 mg/ml concentrations (p <= 0.01). In conclusion, synthesized AuNPs@F. vulgaris revealed non-cytotoxicity, antioxidant, cutaneous wound healing, antibacterial, and antifungal activities.