Ultrastructure of the fertilized egg envelopes in Hemigrammus erythrozonus , Characidae, Teleostei

We examined the morphology and ultrastructures of fertilized egg envelopes of glowlight tetra ( Hemigrammus erythrozonus ) belong to Characidae using light and electron microscopes. The fertilized eggs were spherical, transparent, demersal, adhesive, and have no oil droplet. The perivitelline space was well-developed and the micropyle was surrounded by 15–20 uplifted lines of egg envelope in a spoke like pattern. The outer surface of egg envelope was rough side with grooves. Also, the total thickness of the fertilized egg envelope was about 2.1–2.3 μm, and the fertilized egg envelope consisted of two layers, an outer adhesive electron-dense layer with grooves and three feather-like lamellae layers. Collectively, these morphological characteristics of fertilized egg and micropyle with spoke-like structure showed family Characidae specificity, and ultrastructures of outer surface and section of fertilized egg envelope showed species specificity.