Mesoporous Bioactive Glass Promoted Osteoblast Proliferation and Differentiation In Vitro

This study aimed to evaluate the bioactivity and cytocompatibility of mesoporous bioactive glass. The structure and components of mesoporous bioactive glass 80S MBG were characterized. Mouse osteoblasts were isolated and treated with 80S MBG. The viability and alkaline phosphatase activity of osteoblasts were evaluated. Hydroxyapatite was at the surface of 80S MBG after 4 h, and Ca, P and Si concentration rapidly reached the peak within 1-4 h. pH change was very slight. Mouse osteoblasts seeded in the flask could adhere to the surface and maintain normal morphology. The extract of 80S MBG promoted the proliferation of osteoblasts at lower concentrations, and promoted the differentiation of osteoblasts at higher concentrations. The toxicity gradation of 80S MBG was 0-1. In conclusion, 80S MBG has higher biological activity and good cell compatibility in vitro. The influence for body fluid microenvironment is mild. 80S MBG could be used as a substitute material or scaffold in bone tissue engineering.