Fecundity of diploid and triploid Carassius gibelio (Bloch, 1782) females

Event Abstract Back to Event Fecundity of diploid and triploid Carassius gibelio (Bloch, 1782) females Anna Przybyl1*, Dorota Juchno1, Aleksandra Szabelska1 and Alicja Boron1 1 Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland The Prussian carp C. gibelio (Bloch, 1782) is one of the most invasive fish species in Europe (Grabowska et al. 2010; Kalous et al. 2012) inhabiting almost all available aquatic environments from northern Europe to Asia. Successive spread of this species is possible due to an extraordinary resistance to adverse environmental conditions (Kottelat & Freyhof 2007) and reproductive plasticity as bisexual and asexual reproduction by gynogenesis where the sperm of conspecific or other species are used for induction of eggs development (Jiang et al. 2013; Zhou and Gui 2017). Until the 1990s, the majority of European C. gibelio populations were thought to be almost exclusively composed of triploid (3n = 150-160 chromosomes) females that arose through gynogenesis and represented discrete clonal lineages. In recent years, there is observed transition of C. gibelio European populations from asexual to bisexual. This process is apparent at the ploidy and sex ration that triploid gynogenetic females coexist with diploids (2n=100) of both sexes and occasionally with a different number of triploid males (Tóth et al. 2005; Lusková et al. 2010; Boroń et al. 2011; Jiang et al. 2013; Šimková et al. 2015). The aims of the present, preliminary study, was analysis of eggs size and fecundity as components of reproductive potential of diploid and triploid C. gibelio females. The more so because little such comparative data has been published so far. Samples of C. gibelio were collected before spawning season, May 2014 and 2016 during the commercial catching using nylon nets from diploid-triploid population in Siemianowka Reservoir (52⁰50'N, 21⁰30'E) on the Narew River, the Baltic Sea basin. The fish were treated in accordance with the Act of 15 January 2015 about the protection of animals for scientific and educational purposes and according to LKE license no. 20/2013. The absolute fecundity was estimated according to the number of ready-to-lay eggs in the extracted ovary subsamples using the gravimetric method described by Juchno et al. (2013). The ovary and subsamples were fixed in 4% buffered formalin and weighted. In total, absolute fecundity (Fa) of six diploid and 11 triploid females with average standard length 28.96 cm and body weight 799.64 g were estimated. Size of opaque (full of yolk) eggs collected from fixed ovaries was measured using stereomicroscope with an ocular micrometer with accuracy to 0.005 mm. In total 100 eggs were measured each of 10 females. The histological analysis confirmed that the females were in the pre-spawning period. The relative fecundity (Fr) as the number of eggs per 1 gram of body weight was also counted. All variables were checked for normal distribution. T-student test and Mann-Whitney U test (p<0.05) were used to analyze the effect of ploidy level on absolute fecundity and eggs size of diploid and triploid females. Statistical analyses were performer in Statistica 13 for Windows, StatSoft Inc. The average of eggs size ranged from 0.80 to 1.38 mm and from 1.03 to 1.62 mm, respectively for diploid and triploid females. The mean eggs size of triploids (1.29± 0.078) was significantly higher (p<0.05) then of diploid (1.14 ± 0.086) ones. The absolute fecundity was very variable among individuals and ranged from 73196 to 195476 eggs for diploid and from 60853 to 166220 for triploid females. Whereas, Fr ranged from 171 to 318 and from 84 to 171 eggs for diploid and triploid females, respectively. The average Fa of diploid females as 138440 (SE ± 15942.4) was significantly higher (t = - 2.46, df=8.05, p<0.05) in comparison with Fa of triploid females, 93686 eggs (SE ± 8687.2). The presented results revealed that triploid C. gibelio females produce less number but larger eggs than diploid ones. Acknowledgements We express our special thanks to the Polish Angling Association in Bialystok and Mr. Andrzej Filinowicz for help in collecting fish and Msc Paweł Orych for help in presented analysis. This study was financed by the National Science Centre decision DEC - 2017/25/N/NZ8/00328 and no. 12.610.006-300 of the UWM in Olsztyn, Poland. References Boroń A, Szlachciak J, Juchno D, Grabowska A, Jagusztyn B, Porycka K (2011) Karyotype, morphology, and reproduction ability of the Prussian carp, Carassius gibelio (Actinopterygii: Cypriniformes: Cyprinidae) from unisexual and bisexual populations in Poland. Acta Ichthyologica et Piscatoria 41: 19-28 Grabowska, J., Kotusz, J., & Witkowski, A. (2010). Alien invasive fish species in Polish waters: an overview. Folia Zoologica, 59(1), 73-86. Jiang FF, Wang ZW, Zhou L, Jiang L, ZhangXJ, Apalikova OV, Brykovc VA, Gui JF (2013) High male incidence and evolutionary implications of triploid form in northeast Asia Carassius auratus complex. Molecular Phylogenetics and Evolution 66: 350–359 Juchno D, Boroń A, Kujawa R, Szlachciak J, Szacherski S, Spóz A, Grabowska A. (2013). Comparison of egg and offspring size of karyologically identified spined loach, Cobitis taenia L., and hybrid triploid Cobitis females (Pisces, Cobitidae). Archives of Polish Fisheries, 21(4), 293-299. Kalous L, Bohlen J, Rylková K, Petrtýl M (2012) Hidden diversity within the Prussian carp and designation of a neotype for Carassius gibelio (Teleostei: Cyprinidae) Ichthyological Exploration of Freshwaters 23: 11-18 Kottelat, M. & J. Freyhof. 2007. Handbook of European freshwater fishes. Kottelat, Cornol and Freyhof, Berlin Lusková V, Lusk S, Halačka K, Vetešník L (2010) Carassius auratus gibelio - the most successful invasive fish in waters of the Czech Republik. Russian Journal of Biological Invasions 1: 176-180 Šimková A, Hyršl P, Halačka K, Vetešník L (2015) Physiological and condition-related traits in the gynogenetic-sexual Carassius auratus complex: different investments promoting the coexistence of two reproductive forms? BMC Evolutionary Biology 15: 154 Tóth B, Várkonyi E, Hidas A, Meleg Edwiné E, Váradi L (2005) Genetic analysis of offspring from intra- and interspecific crosses of Carassius auratus gibelio by chromosome and RAPD analysis. Journal of Fish Biology 66: 784-797 Zhou L, Gui JF (2017) Natural and artificial polyploids in aquaculture. Aquaculture and Fisheries 2: 103-111 Keywords: Polyploidy, oocytes size, fecundity, Triploids, Diploids Conference: XVI European Congress of Ichthyology, Lausanne, Switzerland, 2 Sep - 6 Sep, 2019. Presentation Type: Poster Topic: GENETICS, GENOMICS AND PHYSIOLOGY Citation: Przybyl A, Juchno D, Szabelska A and Boron A (2019). Fecundity of diploid and triploid Carassius gibelio (Bloch, 1782) females. Front. Mar. Sci. Conference Abstract: XVI European Congress of Ichthyology. doi: 10.3389/conf.fmars.2019.07.00055 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 May 2019; Published Online: 14 Aug 2019. * Correspondence: Ms. Anna Przybyl, Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Warmian-Masurian, 10-718, Poland, anna.przybyl@uwm.edu.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. 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