Dual infection of a Thai isolate HP-PRRSV and the pdmH1N1 2009 SIV in weanling pigs.
THAI JOURNAL OF VETERINARY MEDICINE(2019)
摘要
After the introduction of the pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in Thai swine farms in 2009, a mild clinical outcome was observed and its role in porcine respiratory disease complex (PRDC) is questionable. A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in Thai swine farms, recently. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma spp. commercial farm were randomly divided into 4 groups; Negative, PRRSV, the SIV and Co-infection groups. Pigs in the PRRSV and Co-infection groups were challenged intranasally with the Thai isolate HP-PRRSV at 0 day post HP-PRRSV challenge (dphc) and pigs in the SIV and Co-infection groups were inoculated intratracheally with the pdmH1N1 SIV at 6 dphc. The 1st and 2nd necropsies were at 2 and 4 days post SIV challenge (dsc). A comparison of the lung lesion scores between the PRRSV and Co-infection groups at 2 dsc showed no statistical difference, but at 4 dsc, the lung lesion scores of the Co-infection group had higher average scores than those of the PRRSV group. Similarly, the SIV viral load in the lung of the Co-infection group a showed higher viral load at 4 dsc, compared to the only SIV-infected group. The results suggest that low pathogenic pdmH1N1 SIV could enhance the lung severity when co-infected with the HP-PRRSV leading to PRDC mimicking the field scenarios.
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关键词
co-infection,highly pathogenic PRRSV,pandemic H1N1 2009,pathogenesis,swine influenza virus
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