Protective Effects Of Diosgenin Against Ultraviolet B (Uvb) Radiation-Induced Inflammation In Human Dermal Fibroblasts

Background and objective: Diosgenin is a steroidal sapogenin known for its pharmacological properties including anti-inflammatory effects. This study was aimed to evaluate the in vitroanti-photoaging and anti-inflammatory effects of diosgenin on ultraviolet B (UVB) radiation-induced damage on human dermal fibroblasts. Materials and Methods:The UVB-induced HDF cells were treated with diosgenin at concentrations 5 and 10 mu M, respectively. Cytotoxicity induced by UVB radiation on pre-treated and post-treated HDF cells were tested. The levels of pro-inflammatory cytokines in HDF cells were determined using ELISA method. Western blot analysis was performed to determine the expressions of inflammatory mediators NF-k beta and MMPs. The concentrations of reduced glutathione (GSH) and malondialdehyde (MDA) were determined in the cell homogenates. Results: Diosgenin significantly (p<0.01) reduced the cytotoxicity induced by UVB on HDF cells. Pro-inflammatory cytokines and expressions of inflammatory mediators were significantly reversed (p<0.01) by diosgenin in a concentration dependent manner. Diosgenin was able to increase the level of GSH and prevent MDA formations. Conclusion:The results proved that diosgenin has protective effects on HDF cells against UVB-induced inflammation. Therefore,this active compound can be recommended for pharmaceutical formulations against UV induced skin inflammation.