Partial Inhibition of the Key Glycolytic Enzyme PFKFB3 in Myeloid Cells Impacts Whole-Body Immune Cell and Liver Metabolism, But Not Atherogenesis

Background and Aims: Macrophages play a central role in atherosclerosis and depend highly on glycolysis for energy metabolism, specifically upon pro-inflammatory stimulation. Blockage of glycolytic enzyme PFKFB3 reduced both glycolysis and inflammation. We hypothesised that myeloid PFKFB3 deficiency reduces glycolysis and subsequent pro-inflammatory activity in vascular and extravascular macrophages, attenuating atherosclerotic burden in vivo.