Validation of ITS-2 rDNA nemabiome sequencing for ovine gastrointestinal nematodes and its application to a large scale survey of UK sheep farms.

Elizabeth Redman,Camila Queiroz,David J Bartley,Michel Levy,Russell W Avramenko,John Stuart Gilleard

Veterinary Parasitology（2019）

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摘要

•ITS-2 rDNA nemabiome sequencing optimised and validated for ovine GIN.•ITS-2 rDNA nemabiome sequencing on eggs, L1 and L3 larvae compared.•ITS-2 rDNA nemabiome sequencing applied as a surveillance tool.•Data on the relative abundance of GI nematode species on 119 UK sheep flocks.

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关键词

Nemabiome,Deep-sequencing,Amplicon assay,ITS-2,PCR bias,Correction factors,Sheep

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