Production Of Of Interleukin 1beta And Interferon Gamma By Bovine Mammary Gland Leukocytes Stimulated By Lipopolysaccharide
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES(2018)
摘要
The aim of this trial was to investigate the production of the pro-inflammatory cytokines interleukin lbeta (IL-1 beta) and interferon gamma (IFN-gamma) produced by bovine mammary gland leukocytes following lipopolysaccharide (LPS) challenge (5 mu g LPS of Escherichia coli in 20 ml of PBS) or PBS (20 ml) as a control. The leukocytes were obtained by mammary gland lavage 1, 2, 3, and 7 days following the stimulation. The concentration levels of these cytokines were analyzed by sandwich ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher average number of leukocytes (P<0.01) was recorded for LPS than for PBS stimulation on day 1. The production of IL-1 beta increased following the stimulation on day 1. After that gradual decline was detected. The IFN-gamma production showed a similar trend. Positive correlation between IL-1 beta and IFN-gamma increased level was found.
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关键词
mastitis, mammary gland, interleukin 1beta, interferon gamma, lipopolysaccharide, dairy cattle
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