Preparation and characterization of an in situ crosslinkable glycol chitosan thermogel for biomedical applications

•A new in situ crosslinkable thermogel system (M/SH-HGC) was successfully developed by mixing two kinds of glycol chitosan thermogels.•The M/SH-HGC thermogels retained not only thermogelling properties but also reactive functionalities for chemical crosslinking.•The M/SH-HGC thermogels exhibited enhanced and tunable physicochemical properties without significant cytotoxicity.