Molecular and Biochemical Identification of Reactive Red-11 Decolorising Klebsiella Oxytoca CMGS-3 Bacterial Strain and Study of Toxic Effect on Plants

The tentatively identified bacterial strain was showed decolorisation of 82.3% in mineral salt of medium with 100mg/L Reactive red-11 without any external nutritional source within 72 hours. Finally optimization done at pH-8, temperature -35ºC and with 0.1 % (1gm/L) yeast extract and dye concentration was 200mg/l and decolorisation confirmed around of 94% within 16 hours of incubation. Further work carried on The identification of bacterial isolate and identified as Klebsiella oxytoca CMGS-3 by16s rRNA technology (Gene bank accession num- KT602858), and various biochemical and sugar utilization tests were done, finally complete decolorisation confirmed by the FT-IR analysis, and green house studies were done to check the toxic effect of dye; here the decolorized and untreated dye used to study toxic effect on crops which were widely grown agriculture crops and CMGS-3 Strain showed better results in germination as well as plant growth. Through these results, it concludes isolate CMGS-3 was the better candidate for the decolorisation of wide range of reactive dyes and it is preserved in IMTECH, (Chandigarh) with the MTCC num-12532 for the usage of waste water treatment.