Single-cell multimodal transcriptomics to study neuronal diversity in human stem cell-derived brain tissue and organoid models

•Cellular reprogramming enables human brain tissue and organoid engineering in vitro.•iPSC disease models are challenged by neuronal type heterogeneity.•Single-cell RNA-sequencing permits detailed and unbiased cell type delineation.•Single-cell analysis is valuable for benchmarking and optimizing tissue engineering.•Multimodal single-cell technologies reveal neuronal types and states.