Selection of adsorbents for recombinant human erythropoietin purification

Jana Adamíková,Łukasz Wiśniewski,Tomáš Molnár,Mária Bartošová,Monika Antošová,Viera Illeová,Gabriela Flores-Ramírez,Ľudovít Škultéty,Milan Polakovič

Separation and Purification Technology（2019）

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摘要

•Several types of protein adsorbents were screened for rhEPO purification.•Adsorbents with anion-exchange ligands were not selective.•Adsorbents with cation-exchange ligands bound rhEPO selectively.•Performance of selected adsorbents was verified in column experiments.

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关键词

Recombinant human erythropoietin,Purification capture step,Multimodal chromatography,Ion-exchange chromatography,Multicomponent adsorption equilibrium

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