Morpho-Structural Peculiarities Of Blood Formed Elements And Clinic-Pathological Characteristics In Women With Uterine (Benign, Malignant) Tumors In Menopausal Age

ObjectiveTo evaluate ongoing alterations that reflect on the structural characteristics of blood formed elements based on the hormonal imbalance and to investigate the ABO system antigens among menopausal women with uterine (benign & malignant) tumors.MethodsBlood specimens from women with benign (n=15), malignant (n=15) uterine tumors, & healthy menopausal women (control, n=15) were used. ELISA kits were used for the quantitative determination of hormones. The blood formed elements ultrastructure observations were conducted by electronic microscope.ResultsCompared to control (33.8±0.7 pg/mL), estradiol level was higher in benign (45.7±0.9 pg/mL) & malignant (70.7±3.7 pg/mL) cases (p<0.001). Similar pattern was noted in testosterone levels [control=0.38±0.03 ng/mL, benign=0.55±0.04 ng/mL (p<0.01), malignant=1.56±0.14 ng/mL (p<0.001)] was higher in malignant cases. In contrast, progesterone levels were decreased in the disease cases [control=0.93±0.05 ng/mL, benign=0.44±0.003 ng/mL, 0.31±0.02 ng/ml (p<0.001)]. The percent ABO system antigens distributions were: O(I) (control=60±10.9, benign=40±10.2, malignant=25±10.2), A(II) (control=30±10.2, benign=55±11.1, malignant=70±10.6), B(III) (control=5±4.8, benign=0±0, malignant=0±0), and AB(IV) (control=5±4.8, benign=5±4.8, malignant=5±4.8) percent. Assessments of morphologic structure of erythrocytes revealed numerous pathological form erythrocytes (Poikilocytosis) as benign, also in case of malignant tumors, particularly target cell (codocytes), hamlet cell, Teardrop cells (dacrocytes), Sickle cells (Drepanocytes) erythrocytes.ConclusionsUsing ELISA and transmembrane electron microscopy, our results demonstrate that in the malignant uterine tumor of menopausal women, quantitative/structural changes occur in blood formed elements indicating ongoing alterations in hormonal imbalance. Assessing these changes in structural characteristics would be useful in examining uterine pathologies and subsequent treatment plans.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.