Therapeutic efficacy of Isometamidium chloride in <em>Trypanosomiasis</em> affected cattle

To evaluate the efficacy of Isometamidium chloride, a total of ten (10) crossbred cattle suffering from surra were taken for this study. Trypanosomiasis was confirmed after microscopic detection of Trypanosoma evansi parasite in blood smears of the affected cattle. Affected cattle were treated with Isometamidium chloride injection (@ 0.5 mg/kg bwt IM) along with supportive therapy. Blood and serum samples collected from affected cattle on day 0 (pre-treatment), 3, 7 and 14 (post-treatment) were analysed for haematological and biochemical parameters respectively. Haematological alterations recorded in affected cattle were decreased Hb (8.36 g/dl ± 0.142), PCV (25.91% ± 0.467) and TEC (4.69 ×106/µL ± 0.132)indicating affected were afflicted with anaemia. The observed Leucopenia was accompanied by Lymphocytopenia, Eosinophilia, Neutrophilia and Thrombocytopenia. Similarly biochemical alterations observed in the affected animals were significantly decreased Glucose (34.81 mg/dl ±0.7), Total Protein (5.47 g/dl ± 0.078), Albumin (1.95 g/dl ± 0.055), Globulin (3.52 g/dl ± 0.043) and increased BUN (15.46 mg/dl ± 0.211), Creatinine (0.78 mg/dl ± 0.011 IU/L), ALT (33.9 ± 0.211 IU/L) and AST (123.2 ± 1.62; Table 2). A gradual disappearance of clinical signs with no parasitemia microscopically was recorded on 3rd day among all the affected cattle, along with a gradual restoration in the altered haemato-biochemical parameters on different post-treatment observation days. Hence, it was inferred that Isometamidium choride is very effective against T evansi infection in cattle.