Screening of pgrs, L-Dopa precursor and polyploidy inducer treatments on protein content of Mucuna Pruriens callus.

An investigation has been conducted to analyse the effect of different concentrations of phytohormones (2, 4-D and kinetin) precursor (Tyrosine, 1.0, 1.5, and 2.0mM) and polyploidy inducer (colchicine 313 μM) treatments on protein content in Mucuna pruriens four weeks old calli. Highest amount of protein accumulated in callus raised on MS+ 3.0mg/l 2, 4-D+0.1 mg/l Kn+1.5mM tyrosine as compared to all other treatments of colchicine and tyrosine individually. It was also noticed that the calli sub cultured after 12 hr treatment of colchicine in suspension media on MS+ 3.0mg/l 2, 4-D showed higher concentrations of protein content as compared to all other treatments of colchicine. It indicates that treatment of tyrosine being a precursor of L-DOPA, led to increased level of protein while colchicine being an anti-mitotic, leads to accumulation of all those proteins, within the cell, which take part in the cell division. The study indicates supplementation of polyploidy inducer for shorter duration (313 μM for 12hr) and combination of phytohormone (3.0mg/l 2, 4-D+0.1 mg/l Kn) with 1.5mM tyrosine may be preferred for increased accumulation of protein content in Mucuna pruriens calli.