Using Lipidomics To Probe Lipid Metabolism

Direct‐infusion electrospray ionization triple quadrupole mass spectrometry method with multiple reaction monitoring (MRM) has been employed to measure Arabidopsis thaliana galactolipids and phospholipids (including monoacyl molecular species, molecular species with oxidized acyl chains, phosphatidic acids (PAs), tri‐and tetra‐galactosyldiacylglycerols (TrGDGs and TeGDGs), head‐group‐acylated galactolipids, and head‐group‐acylated phosphatidylglycerol (acPG)), sulfoquinovosyldiacylglycerols (SQDGs), sphingolipids, di‐ and tri‐acylglycerols (DAGs and TAGs), and sterol derivatives following wounding, cold, and freezing stresses. Changes observed are consistent with activation of lipid oxidizing, hydrolyzing, glycosylating, and acylating activities. Analysis of the lipid changes, their inter‐relationships, and their genetic underpinnings will be discussed.