Evaluation of Dispersive Solid-Phase Extraction (d-SPE) as a Clean-up Step for Phenolic Compound Determination of Myrciaria cauliflora Peel

In this study, dispersive solid-phase extraction (d-SPE) was evaluated as a clean-up step for the determination of phenolic compounds in Myrciaria cauliflora peel using an ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method. For the d-SPE clean-up step, the sorbents, diatomaceous earth (DE), chitosan (CT), and graphitized carbon black (GCB), were evaluated in different proportions. The d-SPE clean-up step efficiency was evaluated through total anthocyanin content (ACC) and spectrophotometric scanning of the extracts. The use of DE with GCB in a ratio of 100:10 ( m / m ) was the best clean-up step condition. The ACC showed an 83% reduction of the amount of anthocyanins, and the scanning spectra confirmed that all extracts submitted to the clean-up step presented pigment reduction. A total of eight compounds were found in M. cauliflora peel, with the highest abundance of ellagic acid (1643 ± 353 mg kg −1 ), gallic acid (252 ± 10 mg kg −1 ), and 4-hydroxybenzoic acid (154 ± 133 mg kg −1 ), indicating the antioxidant potential of the peel, which is frequently considered a by-product of Myrciaria cauliflora fruits.