Multifunctional Tannic Acid (TA) and Lysozyme (Lys) Films Built by Layer by Layer for Potential Application on Implant Coating

A multifunctional (TA/Lys) film, featuring good antioxidant property, fast cell attachment at the initial stage, enhanced osteogenesis, and broad-spectrum antibacterial property, was constructed by the layer-by-layer (LBL) method. The building process was monitored by quartz crystal microbalance with dissipation (QCM-D); the physical properties, such as topography, stiffness in dry and liquid state, and conformation of Lys in the film, were thoroughly characterized. These physical properties were modulated by varying the salt concentration at which the film was constructed. The film not only allows for favorable cell attachment and proliferation of preosteoblasts Mc3t3-E1 but also provides antibacterial property against Gram-positive bacteria, S. aureus and M. lysodeikticus, and Gram-negative bacteria, E. coli. It also displays good antioxidant property, which plays a critical role on fast cell attachment at the initial stage.