Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells

The role of oxidative stress in the pathogenesis of type 2 diabetes (T2D), especially pancreatic beta-cell dysfunction and death, has become apparent in the last two decades. Peroxidase- and catalase-based antioxidant mechanisms are particularly weak in beta-cells and can be easily overwhelmed by excessive production of reactive oxygen and nitrogen species in the course of pathological processes. Recent research has attempted to define in detail the mechanistic aspects of oxidative stress-induced beta-cell dysfunction. Here, we describe the procedures for the measurement of various parameters important to assess oxidative stress in pancreatic beta-cells. Detailed protocols for determination of nitric oxide (NO) production, the glutathione redox status, and general oxidative status in beta-cells are presented in this chapter.