Integration of micronucleus, comet, and Pig-a gene mutation endpoints into rat 15-day repeat-treatment studies: Proof-of-principle with Auramine O.

•Successfully validated the sensitivity of liver micronucleus assay.•First combining liver micronucleus assay with PB micronucleus assay, comet assay and Pig-a assay.•The multiple-endpoint genotoxicity assay largely improved the efficiency of genotoxicity and were more complied with the 3R principle.