One-step pentaplex real-time polymerase chain reaction assay for detection of zika, dengue, chikungunya, West nile viruses and a human housekeeping gene.

Nischay Mishra,James Ng,Jennifer L Rakeman,Michael J Perry,Dominick A Centurioni,Amy B Dean,Adam Price,Riddhi Thakkar, Andreina Garcia Angus,Phillip Williamson,Eric Delwart,Christine Carrington,Nikita Sahadeo,Xiaoyu Che,Thomas Briese,Rafal Tokarz,W Ian Lipkin

Journal of Clinical Virology（2019）

引用 17|浏览108

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摘要

•Accurate diagnosis is needed for clinically indistinguishable arboviral infections.•Establishment of differential and rapid diagnosis of ZIKV, DENV, CHIKV, and WNV.•Simultaneous detection of all ZIKV strains, all four DENV serotypes, CHIKV, WNV.

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关键词

qPCR,Zika virus,Dengue virus,Chikungunya virus,West nile virus,Arboviruses,CII-ArboViroPlex rRT PCR

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