A rapid RT-LAMP assay for the detection of all four lineages of Peste des Petits Ruminants Virus.

Journal of Virological Methods(2019)

引用 13|浏览7
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摘要
•Designed N-gene RT-LAMP using all full-genomes available on Genbank.•The RT-LAMP showed comparable diagnostic sensitivity to the real-time RT-PCR assay.•The limit of detection was estimated between 0.3 to 0.8 TCID50 ml−1.•The RT-LAMP could detect PPRV as early as 4 dpi – before clinical signs were observed.•The RT-LAMP assay can support the global PPR eradication campaign.
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关键词
PPRV,RT-LAMP,Diagnostics,Outbreak,Surveillance
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