Apoptotic Effects Of Drug Targeting Conjugates Containing Different Gnrh Analogs On Colon Carcinoma Cells
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(2019)
Abstract
The wide range of cellular target reactions (e.g., antitumor) of gonadotropin-releasing hormone (GnRH) variants provides the possibility to develop multifunctional GnRH conjugates. The aim of our work was to compare the cytotoxic/apoptotic activity of different GnRH-based, daunorubicin (Dau)-linked conjugates with or without butyrated Lys in position 4 ((4)Lys(Bu)) at a molecular level in a human colorectal carcinoma cell line. Cell viability was measured by impedimetry, cellular uptake and apoptosis were studied by flow cytometry, and the expression of apoptosis-related genes was analyzed by qRT-PCR. The modification with (4)Lys(Bu) resulted in an increased cytotoxic and apoptotic effects and cellular uptake of the GnRH-I and GnRH-III conjugates. Depending on the GnRH isoform and the presence of (4)Lys(Bu), the conjugates could regulate the expression of several apoptosis-related genes, especially tumor necrosis factor (TNF), tumor protein p53 (TP53) and the members of growth-factor signaling. The stronger cytotoxicity of GnRH-I and GnRH-III conjugates containing (4)Lys(Bu) was associated with a stronger inhibitory effect on the expression of growth-factor signaling elements in comparison with their (4)Ser counterparts, in which the upregulation of TP53 and caspases (e.g., CASP9) seemed to play a more important role. We were able to provide further evidence that targeting the GnRH receptor could serve as a successful therapeutic approach in colon cancer, and GnRH-III-[(4)Lys(Bu),(8)Lys(Dau=Aoa)] proved to be the best candidate for this purpose.
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Key words
gonadotropin-releasing hormone,butyrate,conjugation,drug-targeting conjugates,impedimetry,daunorubicin,apoptosis,TP53,TNF,FASL
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