DMSA-Functionalized Mesoporous Alumina with a Highly Capacity for Selective Isolation of Immunoglobulin G.

A novel dimercaptosuccinic acid functionalized mesoporous alumina, shortly as DMSA-MA, is synthesized by the dicarboxylic acid groups of dimercaptosuccinic acid molecules coordination to the Al3+ ions located in the meso-structure. The as-prepared DMSA-MA composites possess a large surface area of 91.17 m2/g, a uniform pore size and a high pore volume of 17.22 nm and 0.23 cm3/g, respectively. DMSA coating of meso-structures significantly enhanced its selectivity for glycoprotein adsorption through powerful hydrophilic binding force, and the maximum adsorption capacity of immunoglobulin G (IgG) can reach 2298.6 mg g-1. The captured IgG could be lightly stripped from the DMSA-MA composites with an elution rate of 98.3% by using 0.5 wt% CTAB solution as the elution reagent. DMSA-MA is further employed as sorbent for the enrichment of IgG heavy chain and light chain from human serum sample, SDS-PAGE assay results showed the obtained IgG with high-purity compared to the standard solution of IgG.