Antioxidant and Protective Effects of Atrina Pectinata Extract.

Atrina pectinata (A. pectinata), called pen shell, is an edible shellfish that adheres to the seabed pointed downward and has a triangular shell reaching about 40 cm in length.In this study, we examined the antioxidant effect of an A. pectinata extract exhibiting various radical scavenging activities. These scavenging activities were evaluated using electron spin resonance. Anti-oxidant activities were also determined using the ferric reducing antioxidant power (FRAP) and the ABTS radical scavenging assays. Lipid peroxidation inhibitory activity was confirmed using ferric thiocyanate and thiobarbituric acid assays. Furthermore, the protective effect of the A. pectinata extract against t-BHP-induced oxidative stress on Chang cells were evaluated using MTT assay and the measurement of reactive oxygen species (ROS). These results showed that the A. pectinata extract have strong radical scavenging activities, and exerts protective effect against oxidative stress through reducing intracellular ROS content of Chang cells.