Structural basis of assembly of the human TCR-CD3 complex.

The alpha beta T cell receptor (TCR), in association with the CD3 gamma epsilon-CD3 delta epsilon-CD3 zeta zeta signalling hexamer, is the primary determinant of T cell development and activation, and of immune responses to foreign antigens. The mechanism of assembly of the TCR-CD3 complex remains unknown. Here we report a cryo-electron microscopy structure of human TCR alpha beta in complex with the CD3 hexamer at 3.7 angstrom resolution. The structure contains the complete extracellular domains and all the transmembrane helices of TCR-CD3. The octameric TCR-CD3 complex is assembled with 1:1:1:1 stoichiometry of TCR alpha beta:CD3 gamma epsilon:CD3 zeta zeta:CD3 zeta zeta. Assembly of the extracellular domains of TCR-CD3 is mediated by the constant domains and connecting peptides of TCR alpha beta that pack against CD3 gamma epsilon-CD3 delta epsilon, forming a trimer-like structure proximal to the plasma membrane. The transmembrane segment of the CD3 complex adopts a barrel-like structure formed by interaction of the two transmembrane helices of CD3 zeta zeta with those of CD3 gamma epsilon and CD3 delta epsilon. Insertion of the transmembrane helices of TCR alpha beta into the barrel-like structure via both hydrophobic and ionic interactions results in transmembrane assembly of the TCR-CD3 complex. Together, our data reveal the structural basis for TCR-CD3 complex assembly, providing clues to TCR triggering and a foundation for rational design of immunotherapies that target the complex.