Evaluation of Antigenic Differences Between Wild and Sabin Vaccine Strains of Poliovirus Using the Pseudovirus Neutralization Test
Scientific reports(2019)
摘要
In the endgame of global polio eradication, serosurveillance is essential to monitor each country’s vulnerability to poliomyelitis outbreaks. Previously, we developed pseudovirus poliovirus (PV) neutralization test (pPNT) with type 1, 2, and 3 PV pseudovirus (PV pv ), which possess a luciferase-encoding PV replicon in the capsids of wild-type strains (PV pv [WT]), showing that pPNT with type 2 and 3 PV pv (WT) but not type 1 shows high correlation with the conventional PV neutralization test (cPNT) performed with vaccine strains. Here, we analyse the antigenicity of PV pv (WT) and PV pv with capsid proteins of Sabin vaccine strains (PV pv [Sabin]) in human serum. Type 2 and 3 PV pv (WT) and PV pv (Sabin) show similar antigenicity in the analysed set of human sera in contrast to type 1 PV pv . The levels of PV pv (Sabin) infection (%), including about 70% of PV pv infection (%) measured in the presence of human serum diluted to the cPNT titre, serve as the optimal threshold values for pPNT (5% for type 1 and 2, 10% for type 3) to show high correlation with cPNT results. Our results suggest that pPNT with PV pv (Sabin) could serve as an alternative to cPNT and provide a rationale for pPNT threshold values.
更多查看译文
关键词
Applied microbiology,Clinical microbiology,Science,Humanities and Social Sciences,multidisciplinary
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要